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plenti ef1a backbone ng  (Addgene inc)


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    Structured Review

    Addgene inc plenti ef1a backbone ng
    Plenti Ef1a Backbone Ng, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenti+ef1a+backbone+ng/pLenti-EF1a-Backbone(NG)+(Plasmid+%2327963)/pmc11303488-54-26-28
    Average 85 stars, based on 3 article reviews
    plenti ef1a backbone ng - by Bioz Stars, 2026-09
    85/100 stars

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    Related Articles

    Synthesized:

    Article Title: Genetically Engineered CLDN18.2 CAR-T Cells Expressing Synthetic PD1/CD28 Fusion Receptors Produced Using a Lentiviral Vector
    Article Snippet: .. The synthesized DNAs for CT-001 and CT-002 were digested with the restriction enzymes XbaI and XhoI and subcloned into the CARBioV1-MCS vector, which was modified from pLenti-EF1a-Backbone (NG) (Addgene). .. The EZ-FusionTM HT Cloning kit (Enzynomics) was used to subclone the CT-017 construct.

    Article Title: Genetically Engineered CLDN18.2 CAR-T Cells Expressing Synthetic PD1/CD28 Fusion Receptors Produced Using a Lentiviral Vector.
    Article Snippet: .. The synthesized DNAs for CT-001 and CT-002 were digested with the restriction enzymes XbaI and XhoI and subcloned into the CARBioV1-MCS vector, which was modified from pLenti-EF1a-Backbone (NG) (Addgene). .. The EZ-FusionTM HT Cloning kit (Enzynomics) was used to subclone the CT-017 construct.

    Modification:

    Article Title: Genetically Engineered CLDN18.2 CAR-T Cells Expressing Synthetic PD1/CD28 Fusion Receptors Produced Using a Lentiviral Vector
    Article Snippet: .. The synthesized DNAs for CT-001 and CT-002 were digested with the restriction enzymes XbaI and XhoI and subcloned into the CARBioV1-MCS vector, which was modified from pLenti-EF1a-Backbone (NG) (Addgene). .. The EZ-FusionTM HT Cloning kit (Enzynomics) was used to subclone the CT-017 construct.

    Article Title: Genetically Engineered CLDN18.2 CAR-T Cells Expressing Synthetic PD1/CD28 Fusion Receptors Produced Using a Lentiviral Vector.
    Article Snippet: .. The synthesized DNAs for CT-001 and CT-002 were digested with the restriction enzymes XbaI and XhoI and subcloned into the CARBioV1-MCS vector, which was modified from pLenti-EF1a-Backbone (NG) (Addgene). .. The EZ-FusionTM HT Cloning kit (Enzynomics) was used to subclone the CT-017 construct.

    TALENs:

    Article Title: Expanded activity of dimer nucleases by combining ZFN and TALEN for genome editing
    Article Snippet: .. To design TALENs targeting the human HBB gene adjacent to the SCD point mutation, we followed a previously published protocol and obtained the TALE backbone and monomers were obtained from Addgene: pLenti-EF1a-Backbone (NG) (Addgene Plasmid 27963), monomer-NN (Addgene Plasmid 27965), monomer-NI (Addgene Plasmid 27966), monomer-NG (Addgene Plasmid 27967), and monomer-HD (Addgene Plasmid 27968). ..

    Mutagenesis:

    Article Title: Expanded activity of dimer nucleases by combining ZFN and TALEN for genome editing
    Article Snippet: .. To design TALENs targeting the human HBB gene adjacent to the SCD point mutation, we followed a previously published protocol and obtained the TALE backbone and monomers were obtained from Addgene: pLenti-EF1a-Backbone (NG) (Addgene Plasmid 27963), monomer-NN (Addgene Plasmid 27965), monomer-NI (Addgene Plasmid 27966), monomer-NG (Addgene Plasmid 27967), and monomer-HD (Addgene Plasmid 27968). ..

    Article Title: Creating a TALE protein with unbiased 5'-T binding.
    Article Snippet: Contents lists available at ScienceDirect Biochemical and Biophysical Research Communications journal homepage: www.elsevier .com/locate /ybbrc Creating a TALE protein with unbiased 50-T binding 0006-291X/$ - see front matter 2013 Elsevier Inc. All rights reserved. http://dx.doi.org/10.1016/j.bbrc.2013.10.060 ⇑ Corresponding author.. Fax: +81 774 32 3038.. E-mail address: imiki@scl.kyoto-u.ac.jp (M. Imanishi).

    Plasmid Preparation:

    Article Title: Expanded activity of dimer nucleases by combining ZFN and TALEN for genome editing
    Article Snippet: .. To design TALENs targeting the human HBB gene adjacent to the SCD point mutation, we followed a previously published protocol and obtained the TALE backbone and monomers were obtained from Addgene: pLenti-EF1a-Backbone (NG) (Addgene Plasmid 27963), monomer-NN (Addgene Plasmid 27965), monomer-NI (Addgene Plasmid 27966), monomer-NG (Addgene Plasmid 27967), and monomer-HD (Addgene Plasmid 27968). ..

    Article Title: Creating a TALE protein with unbiased 5'-T binding.
    Article Snippet: Contents lists available at ScienceDirect Biochemical and Biophysical Research Communications journal homepage: www.elsevier .com/locate /ybbrc Creating a TALE protein with unbiased 50-T binding 0006-291X/$ - see front matter 2013 Elsevier Inc. All rights reserved. http://dx.doi.org/10.1016/j.bbrc.2013.10.060 ⇑ Corresponding author.. Fax: +81 774 32 3038.. E-mail address: imiki@scl.kyoto-u.ac.jp (M. Imanishi).

    Amplification:

    Article Title: Creating a TALE protein with unbiased 5'-T binding.
    Article Snippet: Contents lists available at ScienceDirect Biochemical and Biophysical Research Communications journal homepage: www.elsevier .com/locate /ybbrc Creating a TALE protein with unbiased 50-T binding 0006-291X/$ - see front matter 2013 Elsevier Inc. All rights reserved. http://dx.doi.org/10.1016/j.bbrc.2013.10.060 ⇑ Corresponding author.. Fax: +81 774 32 3038.. E-mail address: imiki@scl.kyoto-u.ac.jp (M. Imanishi).

    Polymerase Chain Reaction:

    Article Title: Creating a TALE protein with unbiased 5'-T binding.
    Article Snippet: Contents lists available at ScienceDirect Biochemical and Biophysical Research Communications journal homepage: www.elsevier .com/locate /ybbrc Creating a TALE protein with unbiased 50-T binding 0006-291X/$ - see front matter 2013 Elsevier Inc. All rights reserved. http://dx.doi.org/10.1016/j.bbrc.2013.10.060 ⇑ Corresponding author.. Fax: +81 774 32 3038.. E-mail address: imiki@scl.kyoto-u.ac.jp (M. Imanishi).

    Countercurrent Chromatography:

    Article Title: Creating a TALE protein with unbiased 5'-T binding.
    Article Snippet: Contents lists available at ScienceDirect Biochemical and Biophysical Research Communications journal homepage: www.elsevier .com/locate /ybbrc Creating a TALE protein with unbiased 50-T binding 0006-291X/$ - see front matter 2013 Elsevier Inc. All rights reserved. http://dx.doi.org/10.1016/j.bbrc.2013.10.060 ⇑ Corresponding author.. Fax: +81 774 32 3038.. E-mail address: imiki@scl.kyoto-u.ac.jp (M. Imanishi).



    Similar Products

    85
    Addgene inc plenti ef1a backbone ng
    Plenti Ef1a Backbone Ng, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plenti+ef1a+backbone+ng/pLenti-EF1a-Backbone(NG)+(Plasmid+%2327963)/pmc11303488-54-26-28
    Average 85 stars, based on 1 article reviews
    plenti ef1a backbone ng - by Bioz Stars, 2026-09
    85/100 stars
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